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基因,重组蛋白,抗体

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重组鼠SP-D/SFTPD(表面活性蛋白D)
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产品: 浏览次数:977重组鼠SP-D/SFTPD(表面活性蛋白D) 
型号: 50205-M08H
规格: 100µg
单价: 面议
最小起订量:
供货总量:
发货期限: 自买家付款之日起 3 天内发货
有效期至: 长期有效
最后更新: 2010-06-09 16:52
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详细信息

Hemagglutinin (A/California/04/2009(H1N1)) Protein Datasheet

 

HA Protein Price Information

  • 500ug:  Inquiring Price;
  • ≥1mg Bulk:  Inquiring Price

HA Protein Product Information

HA Synonym : HA
Protein Construction:

A DNA sequence encoding the extracellular domain of Influenza A virus (A/California/04/2009(H1N1)) hemagglutinin (ACP41105.1)(Met 1-Gln 529)with cleavage site mutated (IQS→RQRRRRK, HA1+HA2, partially cleaved) was expressed, with a C-terminal polyhistidine tag.

Source: Influenza A H1N1
Expression Host: Human Cells

HA Protein QC Testing

Purity: > 92%, as determined by SDS-PAGE SDS-PAGE:
SDS-PAGE
Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Asp 18
Molecular Mass:

The secreted recombinant Influenza A virus (A/California/04/2009 (H1N1)) HA (mutated) comprises 527 amino acids and predicts a molecular mass of 60 kDa. As a result of glycosylation and proteolysis, the apparent molecular mass of the hemagglutinin is apparoximately 25-30, 50-55 and 80-85 kDa in SDS-PAGE under reducing conditions, corresponding to the HA2, HA1 subunit and the full length HA respectively.

Formulation: Supplied as a 0.2μm filtered solution of PBS, pH7.4

HA Protein Usage Guide

Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.

HA Protein Description

Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans.

V Protein Related Products &Topics

  • HA Protein Related Areas:
  • Proteins:
  • Antibodies:

References

  1. Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
  2. Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
  3. Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
  4. Bonnafous, P. et al., 2000, J. Biol. Chem. 275 : 6160-6166.

This Product Belongs to the Following Categories:

 Influenza Hemagglutinin & Antibody(45)
  H1N1 Hemagglutinin & Antibody (Swine Flu 2009; Brevig 1918 ; Brisbane 2007 )(19)
  Influenza Hemagglutinin Protein
  H1N1 Hemagglutinin Protein

Other Influenza Hemagglutinin Products:

 H5N1 Hemagglutinin & Antibody(26)
 H2N2 Hemagglutinin & Antibody (1)
 H3N2 Hemagglutinin & Antibody(3)
 H7N7 Hemagglutinin & Antibody(1)
 Influenza B Hemagglutinin & Antibody(2)
 Influenza Elisa Kits(2)
 
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